Sony Pictures - Spider-Man

Alison Tomita (voiced by MTV personality, Su Chin Pak) - Main newscaster / reporter on cable news channel, Empire 1 (also know as E-1). E-1 is the choice for news and information by the community. If it's happening within the city, it's on this channel.

Peter Parker (voiced by Neil Patrick Harris) - Alter ego of Spider-Man. He was bitten by a genetically altered spider which gave him superhuman powers. Peter attends college at Empire State University. He works as a stringer photographer for the Daily Bugle as well as selling candid video footage of Spider-Man to Empire 1 to help make ends meet.

Mary Jane Watson / MJ (voiced by Lisa Loeb) - Attends Empire State University where she is majoring in drama. She is independent and free-spirited and is good friends with Peter Parker and Harry Osborn. She struggles with her will they / won't they become romantically involved relationship with Peter Parker on a daily basis. MJ also has feelings for Spider-man not knowing that he is secretly Peter Parker.

Harry Osborn(voiced by Ian Zierling) - Wealthy and privileged friend and roommate of Peter Parker. Attends Empire State University but is not necessarily there for the academics but rather the social life. Blames Spider-Man for his father's death.

Max Dillon / Electro (voiced by Ethan Embry)- Geeky high school friend of Peter Parker who attends Empires State University. When Max is hazed at a fraternity party the prank takes a turn for the worse and ultimately leads to the creation of a villain, Electro. Electro is a high voltage villain that threatens the campus and his former tormentors.

Doc Connors / The Lizard (voiced by Rob Zombie ) - Doc Connors is a world class scientist / professor at Empire State University who is studying reptilian DNA for its regenerative powers. To prove that his theories about the regenerative power of reptilian DNA the Doc secretly experiments on himself. Not only does the serum stimulate regrowth of a previously lost limb but it also has the undesired effect of slowly changing the good doctor into an angry, vengeful Lizard.

J. Jonah Jameson (voiced by Keith Carradine) - Cranky and loud editor of the Daily Bugle. Peter Parker works as a freelance photographer capturing candid photos of Spider-Man for the publication. Jameson often pays far less than the photos are actually worth to Parker.

Cheyenne Tate (voiced by Eve) - Romantic interest of Harry Osborn. She is hiding a secret from Harry and his friends as she is also The Talon, an expert high-tech thief. Caught in the act by Spider-Man, her real identity as Cheyenne is revealed when he removes her goggles. This leaves Peter Parker / Spider-Man with the knowledge that his best friend is involved with a criminal.